Toxin A–Negative, Toxin B–PositiveClostridium difficileInfection Diagnosed by Polymerase Chain Reaction

Detection of the tetanus toxin gene by polymerase chain reaction: a case study.

Chronic Helicobacter cinaedi cellulitis diagnosed by microbial polymerase chain reaction

PCR: polymerase chain reaction TMP-SMX: trimethoprim-sulfamethoxazole XLA: X-linked agammaglobulinemia INTRODUCTION Helicobacter cinaedi is an unusual cause of cellulitis in immunocompromised patients. The organism is fastidious, and blood cultures are often negative, making the diagnosis challenging, especially in those without systemic signs. We report a case of chronic H cinaedi cellulitis i...

Invasive pulmonary aspergillosis diagnosed early by polymerase chain reaction assay.

We compared the usefulness of a polymerase chain reaction (PCR) assay for the early diagnosis of invasive pulmonary aspergillosis with the serodiagnosis of sufficient concentrations of galactomannan using the same serum samples. A patient was treated with prednisolone for the management of hepatitis. Computed tomography (CT) scan of the chest showed the nodular shadow with a cavity containing a...

SPECIFIC AMPLIFICATION OF ASPERGILLUS FUMlGATUS DNA BY POLYMERASE CHAIN REACTION

Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...

Computing by polymerase chain reaction.

A mathematical notation is introduced to represent, at a symbolic level, different mechanisms of DNA recombination, and a 'PCR lemma' is proven by analytically describing the combinatorial properties of the polymerase chain reaction process. This approach led to the discovery of novel techniques, based on a form of PCR which we called cross pairing PCR (briefly XPCR). They were mathematically a...